Kishore P, Wittich R M, Walter R D, Shukla O P
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Microbios. 1993;73(294):7-21.
1,3-Diaminopropane has been identified as the major polyamine of Acanthamoeba culbertsoni. N-acetylputrescine and spermidine were present in appreciable amounts and putrescine as well as N-acetylspermidine were also detected, but spermine was absent. Changes in polyamine levels were observed during the growth of amoebae. Ornithine decarboxylase activity was detected in cell-free extracts but there was very low activity of arginine and lysine decarboxylases. A potent polyamine oxidase was demonstrated which preferentially acted on N8-acetyl-spermidine as the substrate while N1-acetylspermidine was a poor substrate; free polyamines did not serve as a good substrate for this enzyme. Active uptake of polyamines by the amoebae was also demonstrated.
1,3 - 二氨基丙烷已被确定为库氏棘阿米巴的主要多胺。N - 乙酰腐胺和亚精胺含量可观，腐胺以及N - 乙酰亚精胺也被检测到，但精胺不存在。在阿米巴生长过程中观察到多胺水平的变化。在无细胞提取物中检测到鸟氨酸脱羧酶活性，但精氨酸和赖氨酸脱羧酶的活性非常低。证实存在一种高效的多胺氧化酶，它优先作用于N8 - 乙酰亚精胺作为底物，而N1 - 乙酰亚精胺是一种较差的底物；游离多胺不是该酶的良好底物。还证实阿米巴对多胺有主动摄取作用。
