Kim B G, Sobota A, Bitonti A J, McCann P P, Byers T J
Department of Microbiology, Ohio State University, Columbus 43210.
J Protozool. 1987 Aug;34(3):278-84. doi: 10.1111/j.1550-7408.1987.tb03175.x.
Five polyamines which could be separated by high performance liquid chromatography were found in Acanthamoeba castellanii (strain Neff). These included in order of decreasing abundance: 1,3-diaminopropane, spermidine, spermine, norspermidine, and putrescine. Only diaminopropane and norspermidine had been found previously. Spermine was present in cultures grown in broth, but not in defined medium. Radioactive substrates were used to establish that putrescine was synthesized by decarboxylation of ornithine, ornithine was synthesized from arginine or citrulline, and diaminopropane was synthesized from spermidine. The presence of ornithine decarboxylase (EC 4.1.1.17), arginase (EC 3.5.3.1), and urease (EC 3.5.1.5) and the absence of arginine decarboxylase (EC 4.1.1.19) were established. A scheme for polyamine biosynthesis in A. castellanii is proposed.
在卡氏棘阿米巴（Neff株）中发现了五种可用高效液相色谱法分离的多胺。按丰度递减顺序排列，它们分别是：1,3 - 二氨基丙烷、亚精胺、精胺、降亚精胺和腐胺。之前仅发现了二氨基丙烷和降亚精胺。精胺存在于肉汤培养的菌液中，但在限定培养基中不存在。使用放射性底物确定腐胺由鸟氨酸脱羧合成，鸟氨酸由精氨酸或瓜氨酸合成，二氨基丙烷由亚精胺合成。确定了鸟氨酸脱羧酶（EC 4.1.1.17）、精氨酸酶（EC 3.5.3.1）和脲酶（EC 3.5.1.5）的存在以及精氨酸脱羧酶（EC 4.1.1.19）的缺失。提出了卡氏棘阿米巴多胺生物合成的方案。
