POU结构域基因Oct-11的克隆、染色体定位及表达模式
Cloning, chromosomal localization and expression pattern of the POU domain gene Oct-11.
作者信息
Goldsborough A S, Healy L E, Copeland N G, Gilbert D J, Jenkins N A, Willison K R, Ashworth A
机构信息
Chester Beatty Laboratories, Institute of Cancer Research, London, UK.
出版信息
Nucleic Acids Res. 1993 Jan 11;21(1):127-34. doi: 10.1093/nar/21.1.127.
POU domain genes encode a family of highly conserved transacting factors that influence the transcriptional activity of several cell type-specific and ubiquitous genes. We have cloned and sequenced cDNAs encoding a novel mouse POU domain protein, Oct-11, that is closely related within the POU domain to the POU class II proteins, Oct-1 and Oct-2. Recombinant Oct-11 protein binds specifically to an octamer sequence in vitro. The Oct-11 gene is expressed during mouse embryogenesis and in the adult thymus and testis. In addition, it is abundant in the myeloma cell line P3/NS-1/1-Ag4.1. We describe the structure of Oct-11 and its chromosomal localization, and discuss the evidence that the POU class II gene family has evolved by duplication and divergence of a common ancestral gene.
POU结构域基因编码一类高度保守的反式作用因子，这些因子会影响多个细胞类型特异性基因和普遍存在的基因的转录活性。我们已经克隆并测序了编码一种新型小鼠POU结构域蛋白Oct-11的cDNA，该蛋白在POU结构域内与POU II类蛋白Oct-1和Oct-2密切相关。重组Oct-11蛋白在体外能特异性结合八聚体序列。Oct-11基因在小鼠胚胎发育过程中以及成年胸腺和睾丸中表达。此外，它在骨髓瘤细胞系P3/NS-1/1-Ag4.1中含量丰富。我们描述了Oct-11的结构及其染色体定位，并讨论了POU II类基因家族通过共同祖先基因的复制和分化而进化的证据。
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