Hawley R G, Shulman M J, Murialdo H, Gibson D M, Hozumi N
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7425-9. doi: 10.1073/pnas.79.23.7425.
The kappa light chain genes from two mutant hybridoma cell lines defective in kappa light chain synthesis were isolated and compared to the wild-type kappa light chain gene. In each case, the mutant kappa light chain genes were found to contain repetitive DNA elements in their intervening sequences that were not present in the intervening sequences of the wild-type kappa light chain gene. These elements were found to be related to the genes of intracisternal A particles. These results suggest that the decreased production of kappa light chain in the mutant cell lines is due to the presence of the intracisternal A particle-related genes.
从两个在κ轻链合成方面有缺陷的突变杂交瘤细胞系中分离出κ轻链基因，并与野生型κ轻链基因进行比较。在每种情况下，都发现突变的κ轻链基因在其间隔序列中含有野生型κ轻链基因间隔序列中不存在的重复DNA元件。这些元件被发现与池内A颗粒的基因有关。这些结果表明，突变细胞系中κ轻链产量的降低是由于存在与池内A颗粒相关的基因。
