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A simplified and reliable protocol for plasmid DNA sequencing: fast miniprep and denaturation.

作者信息

Yie Y, Wei Z, Tien P

机构信息

Institute of Microbiology, Academia Sinica, Beijing, China.

出版信息

Nucleic Acids Res. 1993 Jan 25;21(2):361. doi: 10.1093/nar/21.2.361.

DOI:10.1093/nar/21.2.361
PMID:8441647
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC309121/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd63/309121/1d01ad922b56/nar00051-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd63/309121/1d01ad922b56/nar00051-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd63/309121/1d01ad922b56/nar00051-0182-a.jpg

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Platon: identification and characterization of bacterial plasmid contigs in short-read draft assemblies exploiting protein sequence-based replicon distribution scores.柏拉图：利用基于蛋白质序列的复制子分布分数，在短读长草图组装中鉴定和描述细菌质粒片段。
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Optimization of protocol for sequencing of difficult templates.难测序模板测序方案的优化
J Biomol Tech. 2010 Jul;21(2):97-102.
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Fundamentals of sequencing of difficult templates--an overview.困难模板测序的基本原理——概述

本文引用的文献

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A rapid boiling method for the preparation of bacterial plasmids.一种制备细菌质粒的快速煮沸法。
Anal Biochem. 1981 Jun;114(1):193-7. doi: 10.1016/0003-2697(81)90473-5.
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A rapid and efficient 'miniprep' for isolation of plasmid DNA.一种用于分离质粒DNA的快速高效“小量制备法”。
Nucleic Acids Res. 1989 May 11;17(9):3604. doi: 10.1093/nar/17.9.3604.
J Biomol Tech. 2006 Jul;17(3):207-17.
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Sequences and expression of pyruvate dehydrogenase genes from Pseudomonas aeruginosa.铜绿假单胞菌丙酮酸脱氢酶基因的序列与表达
J Bacteriol. 1997 Jun;179(11):3561-71. doi: 10.1128/jb.179.11.3561-3571.1997.
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Growth- and substrate-dependent transcription of the formate dehydrogenase (fdhCAB) operon in Methanobacterium thermoformicicum Z-245.嗜热甲酸甲烷杆菌Z-245中甲酸脱氢酶（fdhCAB）操纵子的生长及底物依赖性转录
J Bacteriol. 1997 Feb;179(3):899-908. doi: 10.1128/jb.179.3.899-908.1997.
6
Isolation and characterization of a microspore-specific gene from tobacco.烟草小孢子特异性基因的分离与鉴定
Plant Mol Biol. 1996 May;31(2):213-25. doi: 10.1007/BF00021785.
7
Cloning, sequencing, and growth phase-dependent transcription of the coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase-encoding genes from Methanobacterium thermoautotrophicum delta H and Methanopyrus kandleri.嗜热自养甲烷杆菌δH和坎氏甲烷球菌中依赖辅酶F420的N5,N10-亚甲基四氢甲蝶呤还原酶编码基因的克隆、测序及生长阶段依赖性转录
J Bacteriol. 1995 Dec;177(24):7238-44. doi: 10.1128/jb.177.24.7238-7244.1995.
8
Isolation and sequence analysis of cDNAs encoding phosphoenolpyruvate carboxykinase from the PCK-type C4 grass Urochloa panicoides.从PCK型C4禾本科植物类黍尾稃草中分离和测序编码磷酸烯醇式丙酮酸羧激酶的cDNA
Plant Mol Biol. 1995 Jan;27(2):365-76. doi: 10.1007/BF00020190.
9
Sequence analysis of the nucleoprotein genes of three enterotropic strains of murine coronavirus.三种嗜肠性小鼠冠状病毒核蛋白基因的序列分析
Arch Virol. 1995;140(3):571-9. doi: 10.1007/BF01718432.
10
Organization and growth phase-dependent transcription of methane genes in two regions of the Methanobacterium thermoautotrophicum genome.嗜热自养甲烷杆菌基因组两个区域中甲烷基因的组织及生长阶段依赖性转录
J Bacteriol. 1995 May;177(9):2460-8. doi: 10.1128/jb.177.9.2460-2468.1995.