在测序方案中，测序试剂自身对碱性变性质粒DNA的直接中和作用。 - Suppr

Suppr

超能文献

Direct neutralization of alkaline-denatured plasmid DNA in sequencing protocol by the sequencing reagent itself.

作者信息

Rouer E

机构信息

ICGM, INSERM U-332, Paris, France.

出版信息

Nucleic Acids Res. 1994 Nov 11;22(22):4844. doi: 10.1093/nar/22.22.4844.

DOI:10.1093/nar/22.22.4844

PMID:7984440

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC308541/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8234/308541/885e03f1da76/nar00046-0302-a.jpg

相似文献

Direct neutralization of alkaline-denatured plasmid DNA in sequencing protocol by the sequencing reagent itself.在测序方案中，测序试剂自身对碱性变性质粒DNA的直接中和作用。

Nucleic Acids Res. 1994 Nov 11;22(22):4844. doi: 10.1093/nar/22.22.4844.

Detergent-enhanced sequencing of heat-denatured plasmid DNA.去污剂增强热变性质粒DNA的测序

Biotechniques. 1995 Oct;19(4):554-6.

Rapid plasmid DNA sequencing with multiple octamer primers.使用多个八聚体引物进行快速质粒DNA测序。

Biotechniques. 1994 Apr;16(4):645-6; 648-50.

A more robust, rapid alkaline denaturation sequencing method.一种更稳健、快速的碱性变性测序方法。

Biotechniques. 1993 Feb;14(2):168-9.

Direct sequencing of denatured plasmid DNA.变性质粒DNA的直接测序

Methods Enzymol. 1987;152:556-62. doi: 10.1016/0076-6879(87)52061-4.

Degradation of persistent RNA in RNase-containing, high-throughput alkaline lysis DNA preparations.

Biotechniques. 2000 Aug;29(2):250-2. doi: 10.2144/00292bm10.

Plasmid sequencing.质粒测序

Methods Mol Biol. 1993;23:137-40. doi: 10.1385/0-89603-248-5:137.

A simplified and reliable protocol for plasmid DNA sequencing: fast miniprep and denaturation.一种用于质粒DNA测序的简化且可靠的方案：快速小量制备与变性

Nucleic Acids Res. 1993 Jan 25;21(2):361. doi: 10.1093/nar/21.2.361.

Improved recombinant PCR mutagenesis procedure that uses alkaline-denatured plasmid template.改进的使用碱性变性质粒模板的重组PCR诱变程序。

Biotechniques. 1995 Mar;18(3):376-8.

Direct colony sequencing of plasmid DNA by dye terminator methods.采用染料终止法对质粒DNA进行直接菌落测序。

Biotechniques. 2000 Sep;29(3):442-4. doi: 10.2144/00293bm07.

引用本文的文献

A bromodomain-containing protein from tomato specifically binds potato spindle tuber viroid RNA in vitro and in vivo.一种来自番茄的含溴结构域蛋白在体外和体内均可特异性结合马铃薯纺锤块茎类病毒RNA。

J Virol. 2003 Sep;77(17):9685-94. doi: 10.1128/jvi.77.17.9685-9694.2003.

The mouse homolog of FRG1, a candidate gene for FSHD, maps proximal to the myodystrophy mutation on chromosome 8.

Mamm Genome. 1997 Jun;8(6):394-8. doi: 10.1007/s003359900454.

本文引用的文献

Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.超螺旋测序：一种用于质粒DNA测序的快速简便方法。

DNA. 1985 Apr;4(2):165-70. doi: 10.1089/dna.1985.4.165.

Optimal conditions for supercoil DNA sequencing with the Escherichia coli DNA polymerase I large fragment.

Gene Anal Tech. 1988 Mar-Apr;5(2):32-9. doi: 10.1016/0735-0651(88)90024-6.

Structure of the human lck gene: differences in genomic organisation within src-related genes affect only N-terminal exons.

Gene. 1989 Dec 7;84(1):105-13. doi: 10.1016/0378-1119(89)90144-3.

DNA sequencing with chain-terminating inhibitors.使用链终止抑制剂的DNA测序。

Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7. doi: 10.1073/pnas.74.12.5463.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验