在测序方案中，测序试剂自身对碱性变性质粒DNA的直接中和作用。 - Suppr

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超能文献

Direct neutralization of alkaline-denatured plasmid DNA in sequencing protocol by the sequencing reagent itself.

作者信息

Rouer E

机构信息

ICGM, INSERM U-332, Paris, France.

出版信息

Nucleic Acids Res. 1994 Nov 11;22(22):4844. doi: 10.1093/nar/22.22.4844.

DOI:10.1093/nar/22.22.4844

PMID:7984440

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC308541/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8234/308541/885e03f1da76/nar00046-0302-a.jpg

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本文引用的文献

Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.超螺旋测序：一种用于质粒DNA测序的快速简便方法。

DNA. 1985 Apr;4(2):165-70. doi: 10.1089/dna.1985.4.165.

Optimal conditions for supercoil DNA sequencing with the Escherichia coli DNA polymerase I large fragment.

Gene Anal Tech. 1988 Mar-Apr;5(2):32-9. doi: 10.1016/0735-0651(88)90024-6.

Structure of the human lck gene: differences in genomic organisation within src-related genes affect only N-terminal exons.

Gene. 1989 Dec 7;84(1):105-13. doi: 10.1016/0378-1119(89)90144-3.

DNA sequencing with chain-terminating inhibitors.使用链终止抑制剂的DNA测序。

Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7. doi: 10.1073/pnas.74.12.5463.

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