免疫球蛋白转换区重组位点的DNA序列。
DNA sequences at immunoglobulin switch region recombination sites.
作者信息
Dunnick W, Hertz G Z, Scappino L, Gritzmacher C
机构信息
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620.
出版信息
Nucleic Acids Res. 1993 Feb 11;21(3):365-72. doi: 10.1093/nar/21.3.365.
Abstract
The immunoglobulin heavy chain switch from synthesis of IgM to IgG, IgA or IgE is mediated by a DNA recombination event. Recombination occurs within switch regions, 2-10 kb segments of DNA that lie upstream of heavy chain constant region genes. A compilation of DNA sequences at more than 150 recombination sites within heavy chain switch regions is presented. Switch recombination does not appear to occur by homologous recombination. An extensive search for a recognition motif failed to find such a sequence, implying that switch recombination is not a site-specific event. A model for switch recombination that involves illegitimate priming of one switch region on another, followed by error-prone DNA synthesis, is proposed.
摘要
免疫球蛋白重链从合成IgM转换为IgG、IgA或IgE是由DNA重组事件介导的。重组发生在转换区，即位于重链恒定区基因上游的2 - 10 kb的DNA片段内。本文展示了重链转换区内150多个重组位点的DNA序列汇编。转换重组似乎不是通过同源重组发生的。对识别基序进行广泛搜索未能找到这样的序列，这意味着转换重组不是位点特异性事件。本文提出了一种转换重组模型，该模型涉及一个转换区在另一个转换区上的非法引发，随后是易错的DNA合成。
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