人神经丝轻链基因启动子的功能分析
Functional analysis of the human neurofilament light chain gene promoter.
作者信息
Yazdanbakhsh K, Fraser P, Kioussis D, Vidal M, Grosveld F, Lindenbaum M
机构信息
National Institute for Medical Research, Mill Hill, London, UK.
出版信息
Nucleic Acids Res. 1993 Feb 11;21(3):455-61. doi: 10.1093/nar/21.3.455.
We have carried out a structural and functional analysis on the human NF-L (H-NF-L) gene. It contains a methylation-free island, spanning the 5' flanking sequences and the first exon and a number of neuronal-specific DNase I hypersensitive sites have been identified in the upstream region as well as within the body of the gene. Analysis in cell lines and transgenic mice using a combination of these sites has revealed the presence of a conserved element(s) between -300bp and -190bp which is required for neuronal-specific expression.
我们对人类神经丝轻链（H-NF-L）基因进行了结构和功能分析。它包含一个无甲基化岛，跨越5'侧翼序列和第一个外显子，并且在基因上游区域以及基因内部已鉴定出多个神经元特异性DNase I超敏位点。使用这些位点的组合对细胞系和转基因小鼠进行的分析表明，在-300bp至-190bp之间存在一个保守元件，这是神经元特异性表达所必需的。
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