四甲基联苯胺在血浆血红蛋白测定中的应用。
Use of tetramethylbenzidine in plasma hemoglobin assay.
作者信息
Standefer J C, Vanderjagt D
出版信息
Clin Chem. 1977;23(4):749-51.
PMID:844173
Abstract
Plasma hemoglobin can be assayed by substituting 3,3',5,5'-tetramethylbenzidine for benzidine (a carcinogen) as the chromogenic reducing substance. Sensitivity, precision, and accuracy compare well with methods in which benzidine is used. Plasma inhibition can be accommodated by preincubation with hydrogen peroxide.
摘要
血浆血红蛋白可以通过用3,3',5,5'-四甲基联苯胺替代联苯胺（一种致癌物）作为显色还原物质来进行测定。其灵敏度、精密度和准确度与使用联苯胺的方法相当。通过用过氧化氢预孵育可以消除血浆抑制作用。
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