利用脉冲场凝胶电泳对乳酸乳球菌DRC1乳糖质粒进行稳定性分析。

Stability analysis of the Lactococcus lactis DRC1 lactose plasmid using pulsed-field gel electrophoresis.

作者信息

Ward A C, Hillier A J, Davidson B E, Powell I B

机构信息

Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.

出版信息

Plasmid. 1993 Jan;29(1):70-3. doi: 10.1006/plas.1993.1009.

DOI:10.1006/plas.1993.1009

PMID:8441771

Abstract

Pulsed-field agarose gel electrophoresis of SmaI digests of genomic DNA was used to examine lactose plasmid copy number and stability in Lactococcus lactis. In L. lactis strain DRC1, the plasmid was found to exist as a single-copy plasmid. Transconjugants of strain HID113 carrying this plasmid were unstable. Variants were isolated with improved phenotypic stability resulting from improved maintenance of the lactose plasmid or from integration of part of the plasmid into the lactococcal chromosome.

摘要

采用脉冲场琼脂糖凝胶电泳对基因组DNA的SmaI酶切产物进行分析，以检测乳酸乳球菌中乳糖质粒的拷贝数及稳定性。在乳酸乳球菌DRC1菌株中，该质粒以单拷贝质粒形式存在。携带此质粒的HID113菌株的转接合子不稳定。通过改善乳糖质粒的维持情况或使部分质粒整合到乳球菌染色体中，分离出了表型稳定性提高的变体。