Matsumoto Y, Sarkar G, Sommer S S, Wickner R B
Section on Genetics of Simple Eukaryotes, National Institute of Diabetes, Digestive and Kidney Diseases, N.I.H., Bethesda, MD 20892.
Yeast. 1993 Jan;9(1):43-51. doi: 10.1002/yea.320090106.
SKI8 is a yeast antiviral gene, essential for controlling the propagation of M double-stranded RNA (dsRNA) and thus for preventing virus-induced cytopathology. Our DNA sequence of SKI8 shows that it encodes a 397 amino acid protein containing two copies of a 31 amino acid repeat pattern first identified in mammalian beta-transducin and Cdc4p of yeast. There are also four copies of this repeat in yeast Mak11p, necessary for M dsRNA propagation, and three copies in the putative product of the Dictyostelium AAC3 gene. Analysis of 36 cases of the repeat unit shows they have a consensus predicted structure: N-helix-sheet-turn-sheet-turn-sheet-helix-C.
SKI8是一种酵母抗病毒基因，对于控制M双链RNA（dsRNA）的繁殖至关重要，因此对于预防病毒诱导的细胞病变也很重要。我们的SKI8 DNA序列表明，它编码一种397个氨基酸的蛋白质，该蛋白质包含两个31个氨基酸重复模式的拷贝，这种模式最初在哺乳动物β-转导素和酵母的Cdc4p中被鉴定出来。酵母中M dsRNA繁殖所必需的Mak11p中也有四个这种重复拷贝，而盘基网柄菌AAC3基因的推定产物中有三个拷贝。对36个重复单元的分析表明，它们具有一致的预测结构：N-螺旋-片层-转角-片层-转角-片层-螺旋-C。
