Matsubara S, Shima T, Takamori M
Department of Neurology, Kanazawa University, School of Medicine, Ishikawa, Japan.
Acta Neuropathol. 1993;85(2):138-44. doi: 10.1007/BF00227760.
Experimental allergic myositis (EAM) was produced in SJL/J mice by inoculation with the myosin B fraction of the rabbit skeletal muscle, and the pathological changes were quantified. The myosin B fraction contains actin, myosin, tropomyosin and many other proteins, and has been known to induce severe EAM in guinea pigs. In the present model, macrophages and CD4+ lymphocytes predominated among the infiltrating cells. On the surface of muscle fibers and in the regions of cell infiltration deposition of the immunoglobulin G (IgG) and complement factor 3 was observed. EAM was transferred to normal mice by injecting the serum IgG of EAM. Depleting the recipients of complement before the transfer resulted in less-severe pathological changes. Morphologically, the EAM IgG showed an affinity for the nuclei, myofilaments, sarcolemma and blood vessels of mouse skeletal muscle. Biochemically EAM IgG contained antibodies against myosin, actin, troponin, M protein and other muscle proteins. These results indicated that IgG and complement play important roles in this model.
通过接种兔骨骼肌肌球蛋白B组分在SJL/J小鼠中诱发实验性变应性肌炎（EAM），并对病理变化进行定量分析。肌球蛋白B组分包含肌动蛋白、肌球蛋白、原肌球蛋白和许多其他蛋白质，已知其可在豚鼠中诱发严重的EAM。在本模型中，浸润细胞以巨噬细胞和CD4 +淋巴细胞为主。在肌纤维表面和细胞浸润区域观察到免疫球蛋白G（IgG）和补体因子3的沉积。通过注射EAM血清IgG将EAM转移至正常小鼠。在转移前耗尽受体的补体导致病理变化较轻。形态学上，EAM IgG对小鼠骨骼肌的细胞核、肌丝、肌膜和血管具有亲和力。生化分析显示，EAM IgG含有抗肌球蛋白、肌动蛋白、肌钙蛋白、M蛋白和其他肌肉蛋白的抗体。这些结果表明，IgG和补体在该模型中起重要作用。
