Warnke R, Levy R
J Histochem Cytochem. 1980 Aug;28(8):771-6. doi: 10.1177/28.8.7003003.
Acetone-fixed frozen sections of reactive lymph node, spleen, three B cell lymphomas, and three T cell lymphomas were studied for the presence of Ia-like antigens and two T cell antigens. Detection of the binding of the hybridoma monoclonal antibodies to these antigens took advantage of the biotin-avidin interaction. The detection method employed a biotin conjugate of goat anti-mouse antibody and an avidin conjugate of horseradish peroxidase. B cell lymphoma cells stained for Ia. The cell lymphomas were shown to be heterogeneous for the expression of the two T cell antigens. these three antigens were generally not detectable after 10% formalin fixation of B-5 fixation. Detection of these antigens and the method employed should prove useful in the immunologic categorization of human lymphomas.
对反应性淋巴结、脾脏、三种B细胞淋巴瘤和三种T细胞淋巴瘤的丙酮固定冰冻切片进行研究，以检测Ia样抗原和两种T细胞抗原的存在。利用生物素-抗生物素蛋白相互作用检测杂交瘤单克隆抗体与这些抗原的结合。检测方法采用山羊抗小鼠抗体的生物素缀合物和辣根过氧化物酶的抗生物素蛋白缀合物。B细胞淋巴瘤细胞Ia染色阳性。这三种T细胞淋巴瘤细胞在两种T细胞抗原的表达上显示出异质性。在B-5固定的10%福尔马林固定后，通常无法检测到这三种抗原。这些抗原的检测及所采用的方法应证明对人类淋巴瘤的免疫分类有用。
