Ragland M, Theil E C
Department of Biochemistry, North Carolina State University, Raleigh 27695.
Plant Mol Biol. 1993 Feb;21(3):555-60. doi: 10.1007/BF00028813.
To study how iron-rich nodules concentrate and store iron, ferritin (mRNA, protein) was analyzed in developing soybean nodules and compared to nitrogenase (mRNA/activity) and leghemoglobin (mRNA, protein, heme). Both ferritin mRNA and protein concentrations increased early in nodulation. Later in nodulation ferritin protein declined, in contrast to the mRNA, as nitrogenase (mRNA and activity) increased and leghemoglobin (mRNA and protein) accumulated. A precursor/product relationship between iron stored in ferritin and iron in nitrogenase or leghemoglobin is suggested. The uncoordinated changes in ferritin mRNA and protein during nodulation contrast with nitrogenase mRNA and nitrogenase activity suggesting possible translational and posttranscriptional effects on ferritin expression.
为研究富含铁的根瘤如何浓缩和储存铁，对发育中的大豆根瘤中的铁蛋白（mRNA、蛋白质）进行了分析，并与固氮酶（mRNA/活性）和豆血红蛋白（mRNA、蛋白质、血红素）进行了比较。在结瘤早期，铁蛋白mRNA和蛋白质浓度均增加。在结瘤后期，与mRNA相反，铁蛋白蛋白质减少，此时固氮酶（mRNA和活性）增加，豆血红蛋白（mRNA和蛋白质）积累。这表明铁蛋白中储存的铁与固氮酶或豆血红蛋白中的铁之间存在前体/产物关系。结瘤过程中铁蛋白mRNA和蛋白质的不协调变化与固氮酶mRNA和固氮酶活性形成对比，这表明可能存在对铁蛋白表达的翻译和转录后影响。
