Goji K
Department of Endocrinology and Metabolism, Kobe Children's Hospital, Japan.
J Clin Endocrinol Metab. 1993 Mar;76(3):667-70. doi: 10.1210/jcem.76.3.8445023.
To investigate underlying ultradian periodicities in spontaneous circulating GH concentration, blood samples were drawn from 15 normal short boys every 20 min over a 24-h period, and plasma GH concentrations were measured using an ultrasensitive immunoradiometric assay. The limit of detection for the GH assay was 0.01 microgram/L. The GH time series were analyzed using the Cluster program, Ultra program, cosinor analysis, and autocorrelation analysis. Plasma GH concentrations in 1095 samples derived from 15 normal short boys were all within the detectable range of the assay and ranged from 0.07-52.2 micrograms/L. Thirty-six percent of the GH values in the 1095 samples from 15 normal short boys were below 1 microgram/L, and 82% of them occurred during the diurnal awakening period. Cluster analysis disclosed a total of 176 peaks in 15 normal short boys, with a mean +/- SEM number of significant GH peaks of 12.1 +/- 0.5/24 h. Twelve percent of the 176 peaks were below 1 microgram/L, and 95% of them occurred during the diurnal awakening period. In addition, Cluster analysis disclosed 161 interpulse intervals in total, with a mean +/- SEM interval of 116.5 +/- 4.3 min. The GH interpulse interval did not show a significant 24-h rhythm, whereas the GH peak height increased significantly at night. An independent discrete peak detection program, Ultra, identified 12.6 +/- 0.5 GH peaks/24 h. This result was in good agreement with that from analysis by the Cluster program (P = NS). Autocorrelation analysis revealed that GH time series were significantly autocorrelated in 9 of the 15 boys, with maximal autocorrelation coefficients at 115.5 min, on the average. The mean autocorrelation coefficient for a group of 15 normal short boys was significantly positive at a 100-min lag. These findings suggest that there could be a regularly occurring periodicity of approximately 100-120 min in the human GH time series.
为了研究自发性循环生长激素（GH）浓度潜在的超日周期，在24小时内每隔20分钟从15名正常身材矮小的男孩采集血样，并使用超灵敏免疫放射分析法测量血浆GH浓度。GH检测的检测限为0.01微克/升。使用聚类程序、Ultra程序、余弦分析和自相关分析对GH时间序列进行分析。来自15名正常身材矮小男孩的1095份样本中的血浆GH浓度均在检测范围内，范围为0.07 - 52.2微克/升。15名正常身材矮小男孩的1095份样本中，36%的GH值低于1微克/升，其中82%发生在白天觉醒期。聚类分析显示15名正常身材矮小男孩共有176个峰值，显著GH峰值的平均数量±标准误为12.1±0.5/24小时。176个峰值中有12%低于1微克/升，其中95%发生在白天觉醒期。此外，聚类分析总共显示了161个脉冲间期，平均±标准误间期为116.5±4.3分钟。GH脉冲间期未显示出显著的24小时节律，而GH峰值高度在夜间显著增加。一个独立的离散峰值检测程序Ultra识别出12.6±0.5个GH峰值/24小时。该结果与聚类程序分析结果高度一致（P = 无显著差异）。自相关分析显示，15名男孩中有9名的GH时间序列具有显著的自相关性，平均最大自相关系数出现在115.5分钟。一组15名正常身材矮小男孩的平均自相关系数在滞后100分钟时显著为正。这些发现表明，人类GH时间序列中可能存在约100 - 120分钟的规律性周期性。
