Price M R, Sekowski M, Hooi D S, Durrant L G, Hudecz F, Tendler S J
Cancer Research Laboratories, University of Nottingham, UK.
J Immunol Methods. 1993 Feb 26;159(1-2):277-81. doi: 10.1016/0022-1759(93)90168-7.
Monoclonal antibodies have been prepared against a synthetic peptide with a sequence corresponding to a repeated hydrophilic region of the protein core of the human MUC-2 gastrointestinal mucin. Peptide conjugates, prepared by glutaraldehyde cross-linking with keyhole limpet haemocyanin (KLH) and bovine serum albumin (BSA), were employed as the immunogen and target antigen (for screening by ELISA), respectively. However, for the measurement of antibody binding to peptide by an ELISA procedure, an alternative strategy was developed and is described in this report: peptides were conjugated directly to BSA immobilized by physical adsorption to the surface of microtitre plate wells. This procedure permits peptides to be tested as target antigens by ELISA without prior preparation of peptide-carrier conjugates.
已经制备了针对一种合成肽的单克隆抗体，该合成肽的序列对应于人MUC-2胃肠道粘蛋白核心蛋白的一个重复亲水区。通过戊二醛与钥孔戚血蓝蛋白（KLH）和牛血清白蛋白（BSA）交联制备的肽缀合物分别用作免疫原和靶抗原（用于ELISA筛选）。然而，为了通过ELISA程序测量抗体与肽的结合，开发了一种替代策略并在本报告中进行了描述：将肽直接缀合到通过物理吸附固定在微量滴定板孔表面的BSA上。该程序允许通过ELISA将肽作为靶抗原进行测试，而无需事先制备肽-载体缀合物。
