Huguenot C, Furneaux M T, Thottappilly G, Rossel H W, Hamilton R I
Agriculture Canada Research Station, Vancouver, BC.
J Gen Virol. 1993 Mar;74 ( Pt 3):335-40. doi: 10.1099/0022-1317-74-3-335.
The immunoreactivity of a panel of polyclonal antibodies and monoclonal antibodies (MAbs) raised against African isolates of potyviruses from cowpea and African yam bean was examined in ELISAs. A serological study including reference isolates followed by further characterization in differential hosts resulted in separation of the potyviruses into two distinct serogroups, one containing blackeye cowpea mosaic virus (BlCMV) and the other containing cowpea aphid-borne mosaic virus (CAMV). Using biotin-labelled MAbs, the BlCMV isolates were further subdivided into two serotypes and the CAMV isolates into five serotypes. Because both BlCMV and CAMV induce a very similar mosaic disease in cowpea, different ELISA procedures using mixed MAbs were evaluated and a single protocol was developed which allowed reliable diagnosis of both viruses.
在酶联免疫吸附测定（ELISA）中检测了一组针对来自豇豆和非洲豆薯的非洲马铃薯Y病毒分离株产生的多克隆抗体和单克隆抗体（MAbs）的免疫反应性。一项包括参考分离株的血清学研究，随后在鉴别寄主中进行进一步鉴定，结果将这些马铃薯Y病毒分为两个不同的血清群，一个包含黑眼豇豆花叶病毒（BlCMV），另一个包含豇豆蚜传花叶病毒（CAMV）。使用生物素标记的单克隆抗体，将BlCMV分离株进一步细分为两个血清型，将CAMV分离株细分为五个血清型。由于BlCMV和CAMV在豇豆中都引起非常相似的花叶病，因此评估了使用混合单克隆抗体的不同ELISA程序，并制定了一个单一方案，该方案可以可靠地诊断这两种病毒。
