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非洲豇豆蚜传花叶病毒诊断方法的改进:对豇豆种子检测、育种计划及马铃薯Y病毒属分类的意义

Improved diagnosis of cowpea aphid-borne mosaic virus in Africa: significance for cowpea seed-indexing, breeding programs and potyvirus taxonomy.

作者信息

Huguenot C, Furneaux M T, Clare J A, Hamilton R I

机构信息

Pacific Agriculture Research Centre, Agriculture and Agri-Food Canada.

出版信息

Arch Virol. 1996;141(1):137-45. doi: 10.1007/BF01718594.

DOI:10.1007/BF01718594
PMID:8629941
Abstract

Large-scale surveys in Africa for blackeye cowpea mosaic (B1CMV) and cowpea aphid-borne mosaic (CABMV) showed that several CABMV isolates from Southern Africa were either not or poorly recognized by monoclonal antibodies prepared to isolates collected in West Africa. Selection of three new monoclonal antibodies prepared against the Maputo (Mozambique) isolate of CABMV, and their incorporation into a revised panel of monoclonal antibodies, resulted in the assignment of four of these new CABMV isolates to existing serotypes (II, IV, and V) and three others to a new serotype (VI). The South African isolate of passiflora mosaic virus was shown to be related to CABMV isolates in serotype IV. It is proposed that CABMV isolates be assembled into a distinct species in the legume-infecting, aphid-transmissible potyviruses.

摘要

在非洲针对黑眼豇豆花叶病毒(B1CMV)和豇豆蚜传花叶病毒(CABMV)开展的大规模调查显示,从西非收集的分离株制备的单克隆抗体对来自南部非洲的几种CABMV分离株识别效果不佳或完全无法识别。针对莫桑比克马普托CABMV分离株制备了三种新的单克隆抗体,并将它们纳入修订后的单克隆抗体组,结果将其中四种新的CABMV分离株归为现有血清型(II、IV和V),另外三种归为新血清型(VI)。西番莲花叶病毒的南非分离株被证明与血清型IV中的CABMV分离株有关。建议将CABMV分离株归为豆科感染、蚜虫传播的马铃薯Y病毒属中的一个独特物种。

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Arch Virol. 1996;141(1):137-45. doi: 10.1007/BF01718594.
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Virus Genes. 2000;20(1):71-7. doi: 10.1023/a:1008164324663.

本文引用的文献

1
Evidence that cowpea aphid-borne mosaic and blackeye cowpea mosaic viruses are two different potyviruses.豇豆蚜传花叶病毒和黑眼豇豆花叶病毒是两种不同马铃薯Y病毒的证据。
J Gen Virol. 1993 Mar;74 ( Pt 3):335-40. doi: 10.1099/0022-1317-74-3-335.
2
Cowpea aphid borne mosaic virus-Morocco and South African Passiflora virus are strains of the same potyvirus.豇豆蚜传花叶病毒 - 摩洛哥株系和南非西番莲病毒是同一马铃薯Y病毒属病毒的株系。
Arch Virol. 1994;136(1-2):207-17. doi: 10.1007/BF01538830.
3
Capsid protein properties of cowpea aphid-borne mosaic virus and blackeye cowpea mosaic virus confirm the existence of two major subgroups of aphid-transmitted, legume-infecting potyviruses.
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J Gen Virol. 1994 Dec;75 ( Pt 12):3555-60. doi: 10.1099/0022-1317-75-12-3555.
4
Cloning, sequencing, and expression in Escherichia coli of the coat protein gene of a new potyvirus infection South African Passiflora.一种新的感染南非西番莲的马铃薯Y病毒外壳蛋白基因的克隆、测序及在大肠杆菌中的表达
Arch Virol. 1993;128(1-2):29-41. doi: 10.1007/BF01309786.
5
Taxonomy and classification of legume-infecting potyviruses. A proposal from the Potyviridae Study Group of the Plant Virus Subcommittee of ICTV.感染豆科植物的马铃薯Y病毒属病毒的分类学与分类。国际病毒分类委员会植物病毒小组委员会马铃薯Y病毒科研究组的一项提议。
Arch Virol. 1994;139(1-2):231-5.