Spinule-type neurite outgrowth from horizontal cells during light adaptation in the carp retina: an actin-dependent process.

Dendrites of horizontal cells in the carp retina which invaginate the cone pedicles form numerous spinules during light adaptation. We have analyzed the contribution of cytoskeletal elements to this process. Isolated horizontal cells and frozen sections were screened with phalloidin for the existence of F-actin. F-actin was present in all types of horizontal cells and particularly enriched in the distal parts of the dendrites. Electron microscopical analysis demonstrated that interruption of the F-actin polymerization with cytochalasin B inhibited the formation of spinules during light adaptation. The persistence of spinules was also affected. Cytochalasin B also prevented the light-independent, phorbol ester-induced formation of spinules. Cytochalasin B only affected the morphology of the lateral, spinule-forming dendrites of cone horizontal cells within the cone pedicles, leaving the central, non spinule-forming dendrites of cone horizontal cells and the processes of rod horizontal cells within rod spherules unaffected. Whereas cytochalasin B prevented the protrusion of spinules, the spinule-associated membrane densities were only slightly affected. The two main characteristics of spinules, protrusion and membrane densities are therefore independently regulated processes.