Monasebian D M, Ruskin J D, Pirruccello S J
Department of Oral and Maxillofacial Surgery, University of Nebraska Medical Center, Omaha 68198-3010.
J Oral Maxillofac Surg. 1993 Mar;51(3):292-7. doi: 10.1016/s0278-2391(10)80178-8.
To date, few studies have evaluated the diagnostic and prognostic potential of flow cytometry in oropharyngeal tumors. The purpose of this study was to evaluate the interrelationship of anatomic location with the cytogenetic and cytokinetic characteristics of squamous cell carcinomas of the posterior oropharynx. Forty-eight paraffin-embedded squamous cell carcinoma specimens from the posterior oropharynx in clinical stages I to IV were analyzed with an Epics-C flow cytometer (Coulter Diagnostics, Hialeah, FL). Histopathologic grading was performed by the same pathologist, based on tumor differentiation. Of the 38 specimens suitable for analysis, several correlations were noted. First, as the %S-phase increased, the clinical stage increased. Second, as the histologic grade increased, the aneuploid frequency increased. Finally, as the tumor size increased, the histologic grade increased. From the results of this study, it was concluded that %S-phase may be useful in assessing the prognosis of squamous cell carcinomas of the posterior oropharynx. DNA index or aneuploid frequency, also may have value as a prognostic indicator. Finally, the lack of correlation between TNM staging and histologic grading stems not from tumor size but from nodal and metastatic involvement. Further studies are warranted to better define the usefulness of flow cytometric analysis in squamous cell carcinomas of the oropharyngeal region.
迄今为止，很少有研究评估流式细胞术在口咽肿瘤中的诊断和预后潜力。本研究的目的是评估口咽后部鳞状细胞癌的解剖位置与细胞遗传学和细胞动力学特征之间的相互关系。使用Epics - C流式细胞仪（库尔特诊断公司，佛罗里达州希亚莱市）对48例临床I至IV期口咽后部石蜡包埋的鳞状细胞癌标本进行分析。由同一位病理学家根据肿瘤分化程度进行组织病理学分级。在38例适合分析的标本中，发现了几种相关性。首先，随着S期百分比增加，临床分期增加。其次，随着组织学分级增加，非整倍体频率增加。最后，随着肿瘤大小增加，组织学分级增加。从本研究结果得出结论，S期百分比可能有助于评估口咽后部鳞状细胞癌的预后。DNA指数或非整倍体频率也可能作为预后指标具有价值。最后，TNM分期与组织学分级之间缺乏相关性并非源于肿瘤大小，而是源于淋巴结和转移情况。有必要进行进一步研究以更好地确定流式细胞术分析在口咽区域鳞状细胞癌中的有用性。
