Hemmer J, Kreidler J
Department of Oral and Maxillofacial Surgery, University of Ulm, Federal Republic of Germany.
Cancer. 1990 Jul 15;66(2):317-20. doi: 10.1002/1097-0142(19900715)66:2<317::aid-cncr2820660220>3.0.co;2-x.
The clonal DNA content of tumor biopsy specimens of 110 patients with primary and untreated squamous cell carcinoma of the oral cavity was determined by flow cytometric study. The ploidy status was compared with tumor size and histologic grading. Thirty tumors (27.3%) were diploid; in 80 cases (72.7%) cell lines with abnormal DNA content were detected. The portion of aneuploid tumors increased with decreasing degree of histologic differentiation (P less than 0.001) from G1 (38.1%) to G2 (76.6%) and G3 (92.0%). Only one of 13 T1 carcinomas (7.6%) showed abnormal clonal DNA content, but 76.9% of T2 and 90.6% T3 tumors (P less than 0.001) did. The emergence of aneuploid clones obviously represents a marker of malignancy progression in oral carcinoma.
通过流式细胞术研究确定了110例未经治疗的原发性口腔鳞状细胞癌患者肿瘤活检标本的克隆DNA含量。将倍性状态与肿瘤大小和组织学分级进行比较。30个肿瘤（27.3%）为二倍体；在80例（72.7%）中检测到DNA含量异常的细胞系。非整倍体肿瘤的比例随着组织学分化程度从G1（38.1%）降至G2（76.6%）和G3（92.0%）而增加（P<0.001）。13例T1期癌中只有1例（7.6%）显示克隆DNA含量异常，但T2期肿瘤的76.9%和T3期肿瘤的90.6%显示异常（P<0.001）。非整倍体克隆的出现显然代表了口腔癌恶性进展的一个标志。
