Bertini J, Mannucci C, Noferini R, Perico A, Rovero P
A. Menarini Industrie Farmaceutiche Riunite S.r.l., Analytical Research Department, Firenze, Italy.
J Pharm Sci. 1993 Feb;82(2):179-82. doi: 10.1002/jps.2600820213.
A method for the simultaneous quantitative determination of tryptophan and tyrosine in synthetic peptides by second-order derivative diode-array spectroscopy is reported. The method does not require hydrolysis of the peptides or a derivatization reaction; the sample is dissolved in 0.1 N NaOH and directly scanned between 262 and 264 nm to detect tyrosine and between 304 and 306 nm to detect tryptophan. From these results the peptide content of the synthetic sample can be easily calculated in a very simple and fast way. The results obtained by the described method with several peptides are compared with those obtained by classical high-performance liquid chromatographic analysis of amino acids after peptide hydrolysis. A very good correlation was found both for the tryptophan/tyrosine molar ratio and the peptide content.
报道了一种用二阶导数二极管阵列光谱法同时定量测定合成肽中色氨酸和酪氨酸的方法。该方法不需要对肽进行水解或衍生化反应；将样品溶解在0.1 N NaOH中，直接在262至264 nm之间扫描以检测酪氨酸，在304至306 nm之间扫描以检测色氨酸。根据这些结果，可以非常简单快速地轻松计算出合成样品中的肽含量。将所描述的方法应用于几种肽所获得的结果与肽水解后通过经典高效液相色谱法分析氨基酸所获得的结果进行了比较。发现色氨酸/酪氨酸摩尔比和肽含量均具有很好的相关性。
