Chrastil J
Anal Biochem. 1986 Nov 1;158(2):443-6. doi: 10.1016/0003-2697(86)90573-7.
A new spectrophotometric method for quantitative determination of tryptophan and tyrosine in peptides and proteins is described. It is based on two specific color reactions, the reaction of tryptophan with formaldehyde and the reaction of tyrosine and tryptophan with hydroxylamine and ceric cations. By combination of these two reactions both tyrosine and tryptophan can be determined simultaneously. Tyrosine and/or tryptophan bound in peptides and/or proteins react independently of the rest of the peptide or protein molecule. The method is simple, accurate, and sensitive. Hydrolysis is not necessary.
本文描述了一种用于定量测定肽和蛋白质中色氨酸和酪氨酸的新分光光度法。它基于两种特定的显色反应，即色氨酸与甲醛的反应以及酪氨酸和色氨酸与羟胺和铈离子的反应。通过将这两种反应结合起来，可以同时测定酪氨酸和色氨酸。肽和/或蛋白质中结合的酪氨酸和/或色氨酸的反应与肽或蛋白质分子的其余部分无关。该方法简单、准确且灵敏。无需水解。
