Eiglmeier K, Honoré N, Woods S A, Caudron B, Cole S T
Laboratoire de Génétique Moléculaire Bactérienne, Paris, France.
Mol Microbiol. 1993 Jan;7(2):197-206. doi: 10.1111/j.1365-2958.1993.tb01111.x.
In an attempt to unify the genetic and biological research on Mycobacterium leprae, the aetiological agent of leprosy, a cosmid library was constructed and then ordered by a combination of fingerprinting and hybridization techniques. The genome of M. leprae is represented by four contigs of overlapping clones which, together, account for nearly 2.8Mb of DNA. Several arguments suggest that the gaps between the contigs are small in size and that virtually complete coverage of the chromosome has been obtained. All of the cloned M. leprae genes have been positioned on the contig maps together with the 29 copies of the dispersed repetitive element, RLEP. These have been classified into four groups on the basis of differences in their organization. Several key housekeeping genes were identified and mapped by hybridization with heterologous probes, and the current genome map of this uncultivable pathogen comprises 72 loci.
为了统一对麻风病病原体麻风分枝杆菌的基因和生物学研究，构建了一个黏粒文库，然后通过指纹图谱和杂交技术相结合的方法进行排序。麻风分枝杆菌的基因组由四个重叠克隆的重叠群表示，它们总共占近2.8兆碱基的DNA。有几个论据表明，重叠群之间的间隙尺寸很小，并且实际上已经获得了对染色体的几乎完全覆盖。所有克隆的麻风分枝杆菌基因都已与分散重复元件RLEP的29个拷贝一起定位在重叠群图谱上。根据它们的组织差异，这些已被分为四组。通过与异源探针杂交鉴定并定位了几个关键的管家基因，这种不可培养病原体的当前基因组图谱包括72个基因座。
