Horio T, Kohno M, Takeda T
First Department of Internal Medicine, Osaka City University Medical School, Japan.
Metabolism. 1993 Jan;42(1):94-6. doi: 10.1016/0026-0495(93)90178-q.
We examined the secretion of immunoreactive (ir) atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) in primary cultures of atrial and ventricular cardiocytes from neonatal rats, and also investigated the stimulatory effect of endothelin-1 (ET-1) on ir-ANP and ir-BNP release from these cells. After 2 days of culture, rat atrial and ventricular cardiocytes secreted both ir-ANP and ir-BNP into serum-free medium in a time-dependent manner. The amount of ir-BNP released per 10(5) ventricular cells was approximately 60% to 70% (1.8 +/- 0.5 pg/h, 2.5 +/- 0.4 pg/2 h, 2.6 +/- 0.4 pg/4 h) of that released from atrial cells (2.8 +/- 0.4 pg/h, 3.5 +/- 0.6 pg/2 h, 4.6 +/- 0.6 pg/4 h), although ir-ANP was secreted almost entirely from atrial cells. ET-1 clearly stimulated secretion of both ir-ANP and ir-BNP in atrial and ventricular cardiocytes. These observations indicate that ir-BNP is cosecreted with ir-ANP not only from atrial cardiocytes, but also from ventricular cardiocytes in the rat, and that ET-1 stimulates the secretion of these natriuretic peptides by a direct mechanism, not through a hemodynamic change.
我们检测了新生大鼠心房和心室心肌细胞原代培养物中免疫反应性（ir）心房利钠肽（ANP）和脑利钠肽（BNP）的分泌情况，并研究了内皮素 -1（ET -1）对这些细胞释放ir -ANP和ir -BNP的刺激作用。培养2天后，大鼠心房和心室心肌细胞以时间依赖性方式将ir -ANP和ir -BNP分泌到无血清培养基中。每10⁵个心室细胞释放的ir -BNP量约为心房细胞释放量的60%至70%（分别为1.8±0.5 pg/h、2.5±0.4 pg/2 h、2.6±0.4 pg/4 h，心房细胞释放量分别为2.8±0.4 pg/h、3.5±0.6 pg/2 h、4.6±0.6 pg/4 h），尽管ir -ANP几乎完全由心房细胞分泌。ET -1明显刺激心房和心室心肌细胞中ir -ANP和ir -BNP的分泌。这些观察结果表明，ir -BNP不仅与ir -ANP共同从心房心肌细胞分泌，也从大鼠心室心肌细胞分泌，并且ET -1通过直接机制而非通过血流动力学变化刺激这些利钠肽的分泌。
