脑钠肽与心房钠尿肽共同由猪心肌细胞分泌。

Brain natriuretic peptide is cosecreted with atrial natriuretic peptide from porcine cardiocytes.

作者信息

Iida T, Hirata Y, Takemura N, Togashi K, Nakagawa S, Marumo F

机构信息

Department of Biochemistry, Nihon University School of Medicine, Tokyo, Japan.

出版信息

FEBS Lett. 1990 Jan 15;260(1):98-100. doi: 10.1016/0014-5793(90)80076-u.

DOI:10.1016/0014-5793(90)80076-u

PMID:2137097

Abstract

Using primary cultures of atrial cardiocytes from neonatal pig, the secretion brain natriuretic peptide (BNP) and atrial natriuretic peptide (ANP)-like immunoreactivities (LI) was studied in vitro. Porcine cardiocytes time-dependently secreted both BNP-LI and ANP-LI into medium under a serum-free condition, although the amount of BNP-LI secreted was about one-third that of ANP-LI. Phorbol ester and Ca2+ ionophore had less stimulatory effects on secretion of BNP-LI than that of ANP-LI. Reverse-phase HPLC of the conditioned medium revealed a single major BNP-LI component corresponding to synthetic porcine BNP(1-26). These data suggest that a small molecular weight form BNP, possibly BNP(1-26), is cosecreted with ANP from porcine cardiocytes.

摘要

利用新生猪心房心肌细胞的原代培养物，在体外研究了脑钠肽（BNP）和心房钠尿肽（ANP）样免疫反应性（LI）的分泌。在无血清条件下，猪心肌细胞随时间将BNP-LI和ANP-LI分泌到培养基中，尽管分泌的BNP-LI量约为ANP-LI的三分之一。佛波酯和Ca2+离子载体对BNP-LI分泌的刺激作用比对ANP-LI分泌的刺激作用小。对条件培养基进行反相高效液相色谱分析，发现一个与合成猪BNP(1-26)相对应的主要BNP-LI成分。这些数据表明，一种小分子量形式的BNP，可能是BNP(1-26)，与ANP一起从猪心肌细胞中共同分泌。