Breborowicz A, Witowski J, Knapowski J, Serkes K D, Martis L, Oreopoulos D G
Medical Academy, Poznan, Poland.
Nephron. 1993;63(1):15-20. doi: 10.1159/000187138.
We tested the hypothesis that phosphatidylcholine (PC) molecules present in the dialysis solution may interact with the mesothelial cell membrane and modify its function. In vitro experiments were performed on human mesothelial cells (HMC) in culture. PC decreased proliferation of HMC when used at concentrations of 200 mg/l and higher. PC was also cytotoxic to HMC as measured by the release of lactate dehydrogenase from their cytosol. Cells exposed to PC had a diminished capacity for taking up 86Rb from medium. PC decreased the fibrinolytic properties of HMC and increased their procoagulant activity. Our results suggest that the positive short-term effect of the addition of PC to the dialysis solution (i.e., an increase in ultrafiltration) may be over-shadowed by its deleterious action on HMC membrane.
我们检验了这样一个假设，即透析液中存在的磷脂酰胆碱（PC）分子可能与间皮细胞膜相互作用并改变其功能。对培养的人腹膜间皮细胞（HMC）进行了体外实验。当以200mg/L及更高浓度使用时，PC可降低HMC的增殖。通过从HMC细胞溶质中释放乳酸脱氢酶来测定，PC对HMC也具有细胞毒性。暴露于PC的细胞从培养基中摄取⁸⁶Rb的能力减弱。PC降低了HMC的纤溶特性并增加了其促凝血活性。我们的结果表明，向透析液中添加PC的短期积极作用（即超滤增加）可能会被其对HMC膜的有害作用所掩盖。
