Hou F, Zang Y, Zhang X
Department of Nephrology, Nanfang Hospital, First Military Medical University Guangzhou.
Zhonghua Nei Ke Za Zhi. 1995 May;34(5):326-9.
During CAPD, the peritoneal mesothelial monolayer is directly bathed in dialysate containing 1.50%-4.25% glucose. In this study, we separated and cultured mesothelial cells from human omentulum (HMC) to test the effects of glucose on cell growth and matrix biosynthesis. [3H] thymidine incorporation was significantly inhibited in cells grown in > or = 1.00% glucose, compared with cells grown in 0.10% glucose and RPMI-1640 alone. HMC incubated in RPMI-1640 medium released trace fibronectin (FN) as measured by specific enzyme immunoassay, and FN level in supernatants was significantly increased when HMC grew in the media containing glucose more than 0.50%. Glucose induced inhibition of cell proliferation and increase of FN were time-and dose-dependent. Mannitol also achieved the same results, but its inhibitive effect on HMC proliferation is far less than that of isoosmolar glucose. These studies provide evidence for a multitude of direct effects of high concentration ambient glucose level on HMC growth and matrix biosynthesis, and raise the possibility that long-term exposure of HMC to high concentration glucose may induce disturbance in cell repair and metabolism, thus contributing to the formation of sclcerosing peritonitis in CAPD.
在持续性非卧床腹膜透析（CAPD）期间，腹膜间皮单层直接浸泡在含有1.50%-4.25%葡萄糖的透析液中。在本研究中，我们从人网膜中分离并培养了间皮细胞（HMC），以测试葡萄糖对细胞生长和基质生物合成的影响。与仅在0.10%葡萄糖和RPMI-1640中生长的细胞相比，在≥1.00%葡萄糖中生长的细胞中[3H]胸苷掺入受到显著抑制。通过特异性酶免疫测定法测量，在RPMI-1640培养基中孵育的HMC释放微量纤连蛋白（FN），当HMC在含葡萄糖超过0.50%的培养基中生长时，上清液中的FN水平显著增加。葡萄糖诱导的细胞增殖抑制和FN增加具有时间和剂量依赖性。甘露醇也取得了相同的结果，但其对HMC增殖的抑制作用远小于等渗葡萄糖。这些研究为高浓度环境葡萄糖水平对HMC生长和基质生物合成的多种直接作用提供了证据，并提出了HMC长期暴露于高浓度葡萄糖可能诱导细胞修复和代谢紊乱，从而导致CAPD中硬化性腹膜炎形成的可能性。
