Ekong R M, Robson K J, Baker D A, Warhurst D C
Medical Parasitology Department, London School of Hygiene and Tropical Medicine.
Parasitology. 1993 Feb;106 ( Pt 2):107-15. doi: 10.1017/s0031182000074904.
Homologues of the mammalian multidrug resistance gene have been identified in isolates and clones of Plasmodium falciparum and designated pfmdr1 and pfmdr2. Mutations in pfmdr1 have been associated with chloroquine resistance but confirmation could not be obtained in a genetic cross. We have examined the copy number and expression of pfmdr1 and pfmdr2 in chloroquine-sensitive and -resistant P. falciparum and have found no relationship between the copy number of either gene and chloroquine resistance. However, a marked correlation was seen between levels of mRNA transcribed for each gene and chloroquine resistance. Two transcripts of pfmdr1 were detected, and in the asexual blood cycle an 8 kb transcript appeared first, followed by the appearance of a 7 kb species.
在恶性疟原虫的分离株和克隆中已鉴定出哺乳动物多药耐药基因的同源物，并命名为pfmdr1和pfmdr2。pfmdr1中的突变与氯喹抗性有关，但在遗传杂交中无法得到证实。我们检测了氯喹敏感和抗性恶性疟原虫中pfmdr1和pfmdr2的拷贝数和表达情况，发现这两个基因的拷贝数与氯喹抗性之间均无关联。然而，观察到每个基因转录的mRNA水平与氯喹抗性之间存在显著相关性。检测到pfmdr1的两种转录本，在无性血液周期中，8 kb的转录本首先出现，随后出现7 kb的转录本。
