Vande Berg J S, Rudolph R
San Diego Veterans Administration Medical Center, Ca.
Plast Reconstr Surg. 1993 Apr;91(4):684-92. doi: 10.1097/00006534-199304000-00018.
Fibronectin and actin content were measured with immunochemistry in excised wounds in 18 rats. In six rats, wounds were allowed to granulate and contract. Wounds were grafted with full-thickness skin in six rats and with split-thickness skin grafts in the remaining six. Multiple biopsies were taken for immunochemistry and electron microscopy. Actin and fibronectin content was greatest in ungrafted wounds. This content was reduced in wounds by skin grafting, with full-thickness grafts having a generally more inhibitory effect than split. In grafted wounds, actin was observed primarily in the wound bed, whereas fibronectin was present in both the wound bed and graft. Fibronectin appeared coincidently with actin in split-thickness skin grafts and their wounds. In contrast, full-thickness skin grafts had fibronectin staining before actin. The degree of wound contraction inhibition by different types of skin grafts appears to correlate with the fibronectin and actin staining patterns.
采用免疫化学方法测定了18只大鼠切除伤口中的纤连蛋白和肌动蛋白含量。6只大鼠的伤口任其肉芽形成和收缩。6只大鼠的伤口移植了全厚皮片，其余6只移植了中厚皮片。取多个活检组织进行免疫化学和电子显微镜检查。未移植伤口中的肌动蛋白和纤连蛋白含量最高。皮肤移植后伤口中的这种含量降低，全厚皮片的抑制作用通常比中厚皮片更强。在移植伤口中，肌动蛋白主要见于伤口床，而纤连蛋白在伤口床和移植皮片中均有存在。在中厚皮片及其伤口中，纤连蛋白与肌动蛋白同时出现。相比之下，全厚皮片在肌动蛋白出现之前就有纤连蛋白染色。不同类型皮肤移植对伤口收缩的抑制程度似乎与纤连蛋白和肌动蛋白的染色模式相关。
