Inohara H, Nomura T, Hongyo T, Nakajima H, Kawaguchi T, Fukuda K, Sutoh K, Iwasa T, Matsunaga T
Department of Radiation Biology, Osaka University Medical School, Japan.
Acta Otolaryngol Suppl. 1993;501:107-10. doi: 10.3109/00016489309126228.
Selective inbreeding of C.B17-scid/scid mouse pairs showing undetectable IgG and IgM has been carried out in order to reduce the mortality of mice by early occurrence of thymic lymphocytic leukemia and abnormal lymphocyte clones producing immunoglobulins, both of which inhibit the successful heterotransplantation of normal and neoplastic human tissues. Although the majority of C.B17-scid/scid mice showed undetectable (< 1 microgram/ml) or low level (< or = 25 micrograms/ml) of serum IgG and IgM, some produced abnormally high concentrations of IgG and IgM (> 25 micrograms/ml). The incidence of such mice showing higher levels of IgG was very high at F1 and F2 generation (10/55, 18.2%), but significantly low after the F3 generation (18/446, 4.0%, p << 0.001). Although leukemia incidence was very high at F4 to F5 generations (8/40, 20.0%), death from leukemia was not observed early in life (4-6 months after birth) at F7 to F10 generations (0/36, 0%, p < 0.01) and was very low during the age of 6-10 months after the F8 generation (11/66, 16.7% at F4 and F5 vs 4/93, 4.3% at F8-10), p < 0.01). Scid mice improved by the selective inbreeding will provide an invaluable experimental system for the heterotransplantation of normal and neoplastic human tissues.
对血清 IgG 和 IgM 检测不到的 C.B17-scid/scid 小鼠进行选择性近亲繁殖，以降低小鼠因胸腺淋巴细胞白血病早期发生和产生免疫球蛋白的异常淋巴细胞克隆导致的死亡率，这两种情况均会抑制正常和肿瘤性人类组织的成功异种移植。尽管大多数 C.B17-scid/scid 小鼠血清 IgG 和 IgM 检测不到（<1 微克/毫升）或水平较低（≤25 微克/毫升），但有些小鼠产生的 IgG 和 IgM 浓度异常高（>25 微克/毫升）。此类 IgG 水平较高的小鼠在 F1 和 F2 代的发生率非常高（10/55，18.2%），但在 F3 代之后显著降低（18/446，4.0%，p<<0.001）。尽管白血病发生率在 F4 至 F5 代非常高（8/40，20.0%），但在 F7 至 F10 代未观察到早期（出生后 4 - 6 个月）白血病死亡（0/36，0%，p<0.01），且在 F8 代之后的 6 - 10 个月龄期间非常低（F4 和 F5 代为 11/66，16.7%，F8 - 10 代为 4/93，4.3%），p<0.01）。通过选择性近亲繁殖改良的 scid 小鼠将为正常和肿瘤性人类组织的异种移植提供一个非常有价值的实验系统。
