Robelin J, Picard B, Listrat A, Jurie C, Barboiron C, Pons F, Geay Y
INRA-Theix, laboratoire Croissance et Métabolismes des Herbivores, Saint-Genès-Champanelle, France.
Reprod Nutr Dev. 1993;33(1):25-41. doi: 10.1051/rnd:19930103.
The pattern of expression of different types of myosin and the development of different muscle cell populations were studied in the semitendinosus muscle of cattle from 39 d of gestation to 30 d of post-natal life. Monoclonal antibodies specific to different myosin heavy chains were used. Two cell generations were identified during myogenesis. They appeared successively and were characterized by different patterns of expression of myosins. The first population, which was present from the first stage studied (39 d of gestation), gave rise to type I fibers, which, in the mature animal, express only slow myosin. A second generation became differentiated at about 120 d of fetal life and then developed into type II fibers (IIa, IIb or IIc). The beginning of differentiation was characterized in all the cell populations by the expression of specific types of embryonic or fetal myosins. A comparison of these results with findings from previous works shows a marked similarity between species in the pattern of myogenesis but great differences in the length of the different stages of development. In this respect, myogenesis in cattle closely resembles that in man.
研究了从妊娠39天到出生后30天的牛半腱肌中不同类型肌球蛋白的表达模式以及不同肌肉细胞群体的发育情况。使用了针对不同肌球蛋白重链的单克隆抗体。在肌生成过程中鉴定出两代细胞。它们相继出现，并以不同的肌球蛋白表达模式为特征。第一代细胞从研究的第一阶段（妊娠39天）就存在，产生I型纤维，在成熟动物中，I型纤维仅表达慢肌球蛋白。第二代细胞在胎儿期约120天时开始分化，然后发育成II型纤维（IIa、IIb或IIc）。在所有细胞群体中，分化开始的特征是特定类型的胚胎或胎儿肌球蛋白的表达。将这些结果与先前研究结果进行比较表明，不同物种在肌生成模式上有显著相似性，但在不同发育阶段的时长上有很大差异。在这方面，牛的肌生成与人的肌生成非常相似。
