犊牛和成年牛的牛肌肉卫星细胞：用于培养肉生产的细胞和遗传特征的比较研究。

Bovine muscle satellite cells in calves and cattle: A comparative study of cellular and genetic characteristics for cultivated meat production.

作者信息

Kim Bosung, Ko Deunsol, Choi Seong Ho, Park Sungkwon

机构信息

Sejong University, Department of Food Science and Biotechnology, Seoul, 05006, South Korea.

Chungbuk National University, Department of Animal Science, Cheongju, 28644, South Korea.

出版信息

Curr Res Food Sci. 2023 Jul 5;7:100545. doi: 10.1016/j.crfs.2023.100545. eCollection 2023.

This study compared the cellular and genetic characteristics of bovine skeletal muscle satellite cells (SMSCs) from Hanwoo (a Korean native cattle breed), including calves and mature cattle. SMSCs were isolated using magnetic-activated cell sorting (MACS) from tissue samples of six Hanwoo (three calves and three mature cattle) using the CD29 antibody. Calves' SMSCs exhibited significantly faster growth rates than did those from cattle ( < 0.01), with a doubling time of 2.43 days. Genetic analysis revealed higher MyoD and Pax7 expression in SMSCs from calves during proliferation than in those from mature cattle ( < 0.001). However, FASN and PLAG1 expression levels were higher in mature cattle than in calves during both proliferation and differentiation ( < 0.001). These findings highlight the need for strategies to improve bovine muscle cell growth to produce competitive cultivated meat at a competitive price.

本研究比较了韩牛（一种韩国本土牛品种）包括犊牛和成年牛的骨骼肌卫星细胞（SMSC）的细胞和遗传特征。使用磁珠分选法（MACS），利用CD29抗体从6头韩牛（3头犊牛和3头成年牛）的组织样本中分离出SMSC。犊牛的SMSC生长速度明显快于成年牛的SMSC（<0.01），倍增时间为2.43天。遗传分析显示，增殖期犊牛SMSC中MyoD和Pax7的表达高于成年牛SMSC（<0.001）。然而，在增殖和分化过程中，成年牛的FASN和PLAG1表达水平均高于犊牛（<0.001）。这些发现凸显了需要采取策略来改善牛肌肉细胞生长，以便以有竞争力的价格生产出有竞争力的养殖肉。