一种能结合并抑制凝血酶的DNA适配体展现出一种新的DNA结构基序。
A DNA aptamer which binds to and inhibits thrombin exhibits a new structural motif for DNA.
作者信息
Wang K Y, McCurdy S, Shea R G, Swaminathan S, Bolton P H
机构信息
Chemistry Department, Wesleyan University, Middletown, Connecticut 06459.
出版信息
Biochemistry. 1993 Mar 2;32(8):1899-904. doi: 10.1021/bi00059a003.
The investigation of the three-dimensional structure of the DNA aptamer d(G1G2T3-T4G5G6T7G8T9G10G11T12T13G14G15) which binds to and inhibits thrombin has been carried out by NMR methods. This DNA exhibits a number of long-range NOEs between residues which are not adjacent in sequence, which allowed the determination of the novel tertiary structure adopted. This DNA adopts a highly compact, highly symmetrical structure which consists of two tetrads of guanosine base pairs and three loops. The residues of the tetrads alternate anti-syn-anti-syn. This novel structural motif for DNA may also be relevant to the structure of telomere DNA.
通过核磁共振方法对与凝血酶结合并抑制其活性的DNA适配体d(G1G2T3-T4G5G6T7G8T9G10G11T12T13G14G15)的三维结构进行了研究。该DNA在序列上不相邻的残基之间表现出许多长程核Overhauser效应（NOE），这使得能够确定其采用的新型三级结构。该DNA采用高度紧凑、高度对称的结构，由两个鸟嘌呤碱基对四联体和三个环组成。四联体的残基以反式-顺式-反式-顺式交替排列。这种新型的DNA结构基序可能也与端粒DNA的结构有关。
Zotero 插件