Miura K, Shimazu T, Motoki A, Kanai S, Hirano M, Katoh S
Biological Sciences Department, Toray Research Center, Inc., Kamakura, Japan.
Biochim Biophys Acta. 1993 Mar 20;1172(3):357-60. doi: 10.1016/0167-4781(93)90232-3.
The nucleotide sequence of the psbO gene encoding the extrinsic 33 kDa protein (the Mn-stabilizing protein) from the thermophilic cyanobacterium Synechococcus elongatus was determined. The deduced amino acid sequence consisted of 272 residues; 26 for the signal peptide and 246 for the mature protein. The amino acid sequences of nine proteolytic peptides from the isolated protein completely agreed with the deduced amino acid sequence. Several unique variations of amino acids were found in the primary structure, of which some may be related to the high thermostability of the protein.
测定了嗜热蓝藻细长聚球藻中编码外在33 kDa蛋白（锰稳定蛋白）的psbO基因的核苷酸序列。推导的氨基酸序列由272个残基组成；信号肽有26个残基，成熟蛋白有246个残基。从分离出的蛋白中得到的9个蛋白水解肽的氨基酸序列与推导的氨基酸序列完全一致。在一级结构中发现了几个独特的氨基酸变异，其中一些可能与该蛋白的高热稳定性有关。
