Adams G T, Lintilhac P M
Department of Botany, University of Vermont, Burlington 05405-0086.
Biotech Histochem. 1993 Jan;68(1):3-7. doi: 10.3109/10520299309105569.
A technique was developed for sectioning fresh red spruce foliage (Picea rubens Sarg.) for use in fluorescence microscopy. This allowed rapid examination of mesophyll in 3-5 mm needle sections. Healthy, ozone treated and cold stressed needles were examined to assess the utility of this technique for early detection of damage. Healthy mesophyll cells fluoresced bright red, while injured cells fluoresced yellow-green in ozone treated needles, and yellow-orange green in ozone treated needles, and yellow-orange in frozen needles. Shifts in fluorescence wave-lengths may be useful for early detection of injury to mesophyll before it is evident by standard light or electron microscopy.
已开发出一种用于对新鲜红云杉树叶（红云杉）进行切片的技术，以用于荧光显微镜检查。这使得能够在3 - 5毫米的针叶切片中快速检查叶肉。对健康的、经臭氧处理的和冷胁迫的针叶进行了检查，以评估该技术在早期检测损伤方面的效用。健康的叶肉细胞发出亮红色荧光，而在经臭氧处理的针叶中，受损细胞发出黄绿色荧光，在冷冻针叶中发出黄橙色荧光。荧光波长的变化可能有助于在标准光学或电子显微镜下明显观察到叶肉损伤之前对其进行早期检测。
