Rufer N, Tiercy J M, Speiser D, Helg C, Gratwohl A, Chapuis B, Jeannet M, Roosnek E
Département de médecine, Hôpital Cantonal de Genève, Switzerland.
Bone Marrow Transplant. 1993;11 Suppl 1:20-3.
To characterize the pre-transplant cytotoxic T cell precursor (CTLp) activity of unrelated bone marrow donors towards their HLA-ABDR compatible recipients, we have established T cell clones from the primary (positive) CTLp cultures. Two "perfectly matched" oligo-typed DR/DQ identical combinations and two A/B identical, DR subtype incompatible combinations have been studied in detail. Analysis of the clones shows that in the first two combinations the alloreactivity was directed towards a subtype of A2 (A205) and a subtype of Bw60 respectively. In the other two cases, where class II incompatibilities were present, we have not yet defined the exact specificity. However, because the majority of recipient specific clones were of the CD8 phenotype, it is very likely that also in these two cases differences in class I are being recognized.
为了表征无关骨髓供体对其 HLA - ABDR 相容受者的移植前细胞毒性 T 细胞前体（CTLp）活性，我们从原代（阳性）CTLp 培养物中建立了 T 细胞克隆。对两个“完全匹配”的寡分型 DR/DQ 相同组合以及两个 A/B 相同、DR 亚型不相容的组合进行了详细研究。对克隆的分析表明，在前两个组合中，同种异体反应性分别针对 A2 的一个亚型（A205）和 Bw60 的一个亚型。在存在 II 类不相容性的另外两个案例中，我们尚未确定确切的特异性。然而，由于大多数受者特异性克隆为 CD8 表型，在这两个案例中很可能也识别出了 I 类的差异。
