Busca A, Attisano C, Miniero R, Vassallo E, Amoroso A, Mazzola G, Massaia M
Department of Pediatrics, University of Turin, Turin, Italy.
Panminerva Med. 1995 Sep;37(3):168-70.
A child with Sanfilippo syndrome and 5 potential unrelated marrow donors were typed serologically, tested in mixed lymphocyte reaction and typed by restriction fragment length polymorphism analysis in attempt to find a suitable donor. All donors were found to be identical with the recipient, however, these studies were not conclusive in identifying the best match donor. Therefore, recipient-donor pairs were examined by HLA-DR oligotyping. In addition we have studied the potential of cytotoxic T-lymphocytes precursors (CTL-p) analysis as a means of selection for matched unrelated donors. Low frequencies (1/10(5)) of pretransplant CTL-p correlated with oligotyping identity in all donor-recipient pairs evaluated. In one case oligotyping disclosed a previously unrecognized HLA-DRB1 disparity. This resulted in high frequencies of CTL-p (1/35,000). Based on this experience we can argue that CTL-p analysis may be used as an additional tool for selection of compatible unrelated marrow donors.
对一名患有Sanfilippo综合征的儿童以及5名潜在的非亲属骨髓供者进行了血清学分型、混合淋巴细胞反应检测，并通过限制性片段长度多态性分析进行分型，试图找到合适的供者。结果发现所有供者与受者均匹配，但这些研究在确定最佳匹配供者方面并不具有决定性意义。因此，通过HLA-DR寡核苷酸分型对受者-供者配对进行了检测。此外，我们还研究了细胞毒性T淋巴细胞前体（CTL-p）分析作为选择匹配非亲属供者的一种方法的潜力。在所有评估的供者-受者配对中，移植前CTL-p的低频率（1/10⁵）与寡核苷酸分型匹配相关。在一个病例中，寡核苷酸分型揭示了先前未识别的HLA-DRB1差异。这导致CTL-p的频率很高（1/35,000）。基于这一经验，我们可以认为CTL-p分析可作为选择相容非亲属骨髓供者的一种辅助工具。
