McHugh S M, Lachmann P J, Ewan P W
Molecular Immunopathology Unit, MRC Centre, Cambridge, U.K.
Clin Exp Allergy. 1993 Feb;23(2):137-44. doi: 10.1111/j.1365-2222.1993.tb00309.x.
Proliferative responses and production of IL-2 by peripheral blood mononuclear cells of patients with Dermatophagoides pteronyssinus rhinitis +/- asthma were measured at up to 24 hr intervals from 1-8 days, after stimulation with mitogen (PHA), antigen (SKSD) and specific allergen (D. pteronyssinus). IL-2 was measured in culture supernatants using the murine CTLL cell line bioassay. Compared to non-atopic controls, patients had significantly higher proliferative responses to D. pteronyssinus and SKSD but similar responses to PHA. In patients, maximal proliferative responses to PHA, SKSD and D. pteronyssinus were at 3, 6 and 7 days, respectively. IL-2 levels in response to stimulation with D. pteronyssinus were significantly higher in patients than controls, where only background levels were found (15.4, 7.9-30.7 x 10(-2) U/ml [mean, range] n = 11; vs 2.2, 0.1-8.4 x 10(-2) U/ml, n = 6; P < 0.05). In contrast, IL-2 production to PHA and SKSD, although higher in patients, was not significantly different than controls. These results suggest allergic patients possess a population of T cells expandable by specific allergen which are induced to secrete IL-2.
在1至8天内，每隔24小时测量一次粉尘螨鼻炎伴或不伴哮喘患者外周血单个核细胞在丝裂原（PHA）、抗原（SKSD）和特异性变应原（粉尘螨）刺激后的增殖反应及IL-2产生情况。使用小鼠CTLL细胞系生物测定法检测培养上清液中的IL-2。与非特应性对照相比，患者对粉尘螨和SKSD的增殖反应显著更高，但对PHA的反应相似。在患者中，对PHA、SKSD和粉尘螨的最大增殖反应分别在第3、6和7天出现。患者对粉尘螨刺激的IL-2水平显著高于对照，对照仅检测到背景水平（15.4，7.9 - 30.7×10⁻²U/ml[均值，范围]，n = 11；vs 2.2，0.1 - 8.4×10⁻²U/ml，n = 6；P < 0.05）。相反，患者对PHA和SKSD的IL-2产生虽高于对照，但差异无统计学意义。这些结果表明，过敏患者拥有一群可被特异性变应原扩增并被诱导分泌IL-2的T细胞。
