高分子量碱性磷酸酶作为结直肠癌的肿瘤标志物：两种检测方法的比较

High-molecular-mass alkaline phosphatase as a tumor marker for colorectal cancer: comparison of two test methods.

作者信息

Wei J S, Chung N C, Wei L L, Tzeng W F, Liu T Z, Wang J Y

机构信息

Laboratory of Clinical Enzymology, School of Medical Technology, Chang Gung Medical College, Taiwan, R.O.C.

出版信息

Clin Chem. 1993 Mar;39(3):540-3.

PMID:8448874

Abstract

We measured high-molecular-mass alkaline phosphatase (HiMwALP) in serum samples from patients with colorectal cancer by polyacrylamide gel electrophoresis (PAGE) and by column chromatography on diethylaminoethyl (DEAE)-cellulose. Determination of carcinoembryonic antigen (CEA) by an enzyme immunoassay, a frequently used cancer assessment method, was used for comparison. We studied patients with primary colorectal cancer (n = 72), using others with hemorrhoids (n = 38) for a comparison group. HiMwALP activities twice those of pooled normal sera were adopted as cutoff values. The diagnostic sensitivity of the PAGE method for 72 colorectal cancer patients was 63% vs 36% for the DEAE method and 50% for the CEA method. The diagnostic specificities of the PAGE, DEAE, and CEA methods were 89%, 79%, and 95%, respectively. Using both HiMwALP (PAGE method) and CEA for the detection of primary colorectal cancer increased the sensitivity to 72% but decreased specificity to 87%.

摘要

我们通过聚丙烯酰胺凝胶电泳（PAGE）和二乙氨基乙基（DEAE）纤维素柱色谱法，测定了结肠癌患者血清样本中的高分子量碱性磷酸酶（HiMwALP）。采用酶免疫测定法测定癌胚抗原（CEA），这是一种常用的癌症评估方法，用于比较。我们研究了原发性结肠癌患者（n = 72），并将患有痔疮的其他患者（n = 38）作为对照组。HiMwALP活性为混合正常血清两倍的数值被用作临界值。PAGE法对72例结肠癌患者的诊断敏感性为63%，而DEAE法为36%，CEA法为50%。PAGE法、DEAE法和CEA法的诊断特异性分别为89%、79%和95%。同时使用HiMwALP（PAGE法）和CEA检测原发性结肠癌，可将敏感性提高到72%，但特异性降至87%。