Parrinello N, Arizza V, Cammarata M, Parrinello D M
Institute of Zoology, University of Palermo, Italy.
Dev Comp Immunol. 1993 Jan-Feb;17(1):19-27. doi: 10.1016/0145-305x(93)90012-f.
Hemocytes (effectors) of Ciona intestinalis showed a natural cytotoxic capacity (HCA) when assayed in vitro against erythrocytes (targets). Cytotoxic cells lysed, to a variable extent, rabbit (RE), human (A, B, O), guinea pig, and sheep (SE) erythrocytes. Hemocyte cytotoxic activity (HCA) assayed against SE is a calcium-dependent reaction, occurs rapidly (15-30 min), at 25-37 degrees C over a wide range of pH (5.4-8.0). Assays were carried out using: 1) the medium in which hemocytes were maintained, 2) the soluble portion of hemocyte lysates, and 3) debris prepared from hemocyte lysates. Results suggest that HCA is a cell-mediated process that requires effector-target cell contacts. Anti-SE (calcium-dependent) and anti-RE (calcium-independent) agglutinins were also found in the reaction medium, probably released by hemocytes as a consequence of the in vitro experiments. The occurrence of HCA was independent of any allogeneic reaction between mixed hemocytes. Various levels of cytotoxic activity reveal hemocyte specificity.
在体外针对红细胞（靶细胞）进行检测时，玻璃海鞘的血细胞（效应细胞）表现出天然细胞毒性能力（血细胞介导的细胞毒性作用，HCA）。细胞毒性细胞对兔红细胞（RE）、人红细胞（A、B、O型）、豚鼠红细胞和绵羊红细胞（SE）产生不同程度的裂解作用。针对绵羊红细胞检测的血细胞细胞毒性活性（HCA）是一种钙依赖性反应，在25 - 37摄氏度、pH值范围较宽（5.4 - 8.0）的条件下快速发生（15 - 30分钟）。检测使用了以下材料：1）用于培养血细胞的培养基；2）血细胞裂解物的可溶部分；3）由血细胞裂解物制备的碎片。结果表明，HCA是一个细胞介导的过程，需要效应细胞与靶细胞接触。在反应介质中还发现了抗绵羊红细胞（钙依赖性）和抗兔红细胞（钙非依赖性）凝集素，它们可能是体外实验导致血细胞释放出来的。HCA的发生与混合血细胞之间的任何同种异体反应无关。不同水平的细胞毒性活性揭示了血细胞的特异性。
