Parrinello N, Cammarata M, Arizza V
Laboratory of Marine Immunobiology, University of Palermo, Italy.
Biol Bull. 1996 Jun;190(3):418-25. doi: 10.2307/1543035.
A discontinuous, Percoll density gradient was used to separate hemocyte populations from the hemolymph of Ciona intestinalis. Hemocytes from each band were examined for their frequency, morphology, and cytotoxic activity against rabbit and sheep erythrocytes; results were expressed as a percentage of hemolysis. Statistical analysis revealed that only the "univacuolar" granulocytes from Band 5, which contain a vacuole of refractile material, were cytotoxic. Cytotoxic activity was inhibited by sphingomyelin. For the first time in tunicates, lytic activity against erythrocytes was assessed by an assay based on plaque-forming cells. Plaques of lysis were revealed against rabbit erythrocytes but not against sheep erythrocytes.
采用不连续的Percoll密度梯度从玻璃海鞘的血淋巴中分离血细胞群体。对每个条带中的血细胞进行频率、形态以及对兔和绵羊红细胞的细胞毒性活性检测；结果以溶血百分比表示。统计分析表明，只有来自条带5的“单泡”粒细胞具有细胞毒性，这些粒细胞含有一个折光性物质的液泡。鞘磷脂可抑制细胞毒性活性。在被囊动物中首次通过基于空斑形成细胞的测定法评估对红细胞的裂解活性。发现存在针对兔红细胞的裂解空斑，但不存在针对绵羊红细胞的裂解空斑。
