Grigoriev A V
Genome Analysis Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Genomics. 1993 Feb;15(2):311-6. doi: 10.1006/geno.1993.1062.
Genome mapping by anchoring random clones has recently been the subject of intensive theoretical study. In this paper, differences between published predictions of properties of anchored groups of clones ("contigs") are analyzed and simplifications of the mathematical formulae describing these properties are presented. The theoretical predictions are compared with the experimental results from the physical mapping of the genome of Schizosaccharomyces pombe. Information about the number of genome sections with no anchored clone on them ("oceans") and the number of undetected overlaps between the contigs at a given stage of the experiment is required for the decision to change from the random strategy to that of a directed closure of gaps. We demonstrate that the expected number of oceans can be approximated by the number of groups of clones anchored by a single probe ("singletons"), as can the expected number of undetected overlaps between contigs by the number of contigs containing more than one anchor.
通过锚定随机克隆进行基因组图谱绘制近来一直是深入理论研究的主题。本文分析了已发表的关于锚定克隆群（“重叠群”）特性预测之间的差异，并给出了描述这些特性的数学公式的简化形式。将理论预测与粟酒裂殖酵母基因组物理图谱的实验结果进行了比较。在决定从随机策略转变为定向填补缺口策略时，需要有关没有锚定克隆的基因组片段数量（“海洋”）以及实验给定阶段重叠群之间未检测到的重叠数量的信息。我们证明，海洋的预期数量可以由单个探针锚定的克隆群数量（单一克隆）近似，重叠群之间未检测到的重叠预期数量也可以由包含多个锚定的重叠群数量近似。
