Arratia R, Lander E S, Tavaré S, Waterman M S
Department of Mathematics, University of Southern California, Los Angeles 90089.
Genomics. 1991 Dec;11(4):806-27. doi: 10.1016/0888-7543(91)90004-x.
A complete physical map of the DNA of an organism, consisting of overlapping clones spanning the genome, is an extremely useful tool for genomic analysis. Various methods for the construction of such physical maps are available. One approach is to assemble the physical map by "fingerprinting" a large number of random clones and inferring overlap between clones with sufficiently similar fingerprints. E.S. Lander and M.S. Waterman (1988, Genomics 2:231-239) have recently provided a mathematical analysis of such physical mapping schemes, useful for planning such a project. Another approach is to assemble the physical map by "anchoring" a large number of random clones--that is, by taking random short regions called anchors and identifying the clones containing each anchor. Here, we provide a mathematical analysis of such a physical mapping scheme.
生物体DNA的完整物理图谱由跨越基因组的重叠克隆组成，是基因组分析的极为有用的工具。构建此类物理图谱有多种方法。一种方法是通过对大量随机克隆进行“指纹识别”来组装物理图谱，并推断指纹足够相似的克隆之间的重叠情况。E.S.兰德和M.S.沃特曼（1988年，《基因组学》2:231 - 239）最近对这种物理图谱绘制方案进行了数学分析，这对规划此类项目很有用。另一种方法是通过“锚定”大量随机克隆来组装物理图谱——也就是说，通过获取称为锚的随机短区域并识别包含每个锚的克隆。在此，我们对这种物理图谱绘制方案进行数学分析。
