用于基因图谱绘制的微卫星分析优化
Optimization of microsatellite analysis for genetic mapping.
作者信息
Hughes A E
机构信息
Department of Medical Genetics, Queen's University of Belfast, Belfast City Hospital, United Kingdom.
出版信息
Genomics. 1993 Feb;15(2):433-4. doi: 10.1006/geno.1993.1083.
PMID:8449514
Abstract
A method for typing microsatellite polymorphisms is described. It involves amplification using the polymerase chain reaction with one primer 5' end-labeled with 32P. Alleles are separated by denaturing gel electrophoresis and detected by autoradiography. Standardized conditions allow accurate typing of almost all microsatellite polymorphisms, and results are usually obtained within 24 h.
摘要
描述了一种微卫星多态性分型方法。该方法包括使用聚合酶链反应进行扩增，其中一个引物的5'端用32P标记。通过变性凝胶电泳分离等位基因，并通过放射自显影进行检测。标准化条件可对几乎所有微卫星多态性进行准确分型，通常在24小时内获得结果。
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