Saha M, Giese R W
Department of Pharmaceutical Sciences, College of Pharmacy and Allied Health Professions, Boston, MA.
J Chromatogr. 1993 Feb 12;631(1-2):161-3. doi: 10.1016/0021-9673(93)80515-a.
When a low-nanogram amount of N1,N3-bis-(pentafluorobenzyl)-N7-(2- [pentafluorobenzyloxy]ethyl)xanthine was subjected to HPLC, low-picogram amounts of the compound could be detected subsequently (off-line by gas chromatography-electron-capture negative-ion mass spectrometry) after injection of pure mobile phase. This was in spite of significant, intermediate washing of the injector and column. It was determined that essentially 99.9% of this analyte contamination came from the injector. Use of two injectors is a practical remedy for this problem.
当将低纳克量的N1,N3-双-(五氟苄基)-N7-(2-[五氟苄氧基]乙基)黄嘌呤进行高效液相色谱分析时，在注入纯流动相后，随后（通过气相色谱-电子捕获负离子质谱离线检测）可检测到低皮克量的该化合物。尽管对进样器和色谱柱进行了大量的中间清洗，但仍出现这种情况。经测定，这种分析物污染基本上99.9%来自进样器。使用两个进样器是解决这个问题的一种实际方法。
