Kroviarski Y, Cochet S, Martineau P, Cartron J P, Bertrand O
INSERM U 76, Paris, France.
J Chromatogr. 1993 Feb 24;633(1-2):273-80. doi: 10.1016/0021-9673(93)83163-m.
To obtain affinity adsorbents with good mechanical resistance, suitable for the purification of maltose-binding protein (MBP) from Escherichia coli and genetically engineered proteins fused to MBP, a series of supports were prepared by grafting amylose on to agarose by different chemistries. Their capacities for MBP and their abilities to be used at relatively high flow-rates were examined. Efficient supports were most conveniently prepared by coupling amylose to epoxy-activated agarose in an aqueous-organic mixture.
为了获得具有良好机械抗性的亲和吸附剂，适用于从大肠杆菌中纯化麦芽糖结合蛋白（MBP）以及与MBP融合的基因工程蛋白，通过不同化学方法将直链淀粉接枝到琼脂糖上制备了一系列载体。考察了它们对MBP的结合容量以及在相对较高流速下使用的能力。通过在水-有机混合物中将直链淀粉偶联到环氧活化的琼脂糖上，可以最方便地制备高效载体。
