Srinivasan U, Bell J A
Department of Chemistry, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
J Biotechnol. 1998 Jul 16;62(3):163-7. doi: 10.1016/s0168-1656(98)00058-3.
Maltose binding protein of Escherichia coli is frequently employed as a fusion partner for the biosynthesis of polypeptides and proteins, largely because of the advantage provided by affinity purification of such a fusion. A mixture of cellulose and starch is shown to be a simple and effective alternative to the use of other, more complicated media which are often used for affinity chromatography of maltose binding protein fusions.
大肠杆菌的麦芽糖结合蛋白经常被用作多肽和蛋白质生物合成的融合伴侣，这主要是因为这种融合蛋白在亲和纯化方面具有优势。已证明纤维素和淀粉的混合物是一种简单有效的替代品，可替代其他常用于麦芽糖结合蛋白融合物亲和色谱的更复杂介质。
