Morgan A, Collier C G, Kellington J P
Biomedical Research Department, AEA Environment and Energy, Harwell Laboratory, Oxfordshire, United Kingdom.
J Toxicol Environ Health. 1993 Mar;38(3):245-56. doi: 10.1080/15287399309531716.
The distribution of glass fibers in the peritoneal cavity of the rat was investigated at 2, 24, and 48 h following intraperitoneal injection of 1 mg of material using a radioactive tracer technique. At each time point the peritoneal cavities of the rats killed were lavaged with 20 ml of physiological saline to recover fibers not yet attached to tissue surfaces. At 2 h, 35% of the administered fiber could be recovered by lavage, but at 48 h this was reduced to 2%. At 48 h, the amount of fiber associated with the abdominal organs and the abdominal wall was roughly in proportion to their surface areas. The weight of fiber associated with the various tissues was in the following order: gastrointestinal tract > liver > carcass (abdominal wall) > diaphragm > urogenital tract > spleen > kidneys. Differential counts on cells recovered by lavage were made both on cytocentrifuge slides and by flow cytometry. Compared with controls, the numbers of cells recovered from treated rats at 24 and 48 h were increased by a factor of about 2, due mainly to an influx of neutrophils into the peritoneal cavity. There was a marked reduction in the proportion of mast cells compared to controls.
采用放射性示踪技术，在腹腔注射1毫克物质后的2小时、24小时和48小时，对大鼠腹腔内玻璃纤维的分布情况进行了研究。在每个时间点，用20毫升生理盐水冲洗处死大鼠的腹腔，以回收尚未附着在组织表面的纤维。在2小时时，通过冲洗可回收35%的给药纤维，但在48小时时，这一比例降至2%。在48小时时，与腹部器官和腹壁相关的纤维量大致与其表面积成比例。与各种组织相关的纤维重量顺序如下：胃肠道＞肝脏＞躯体（腹壁）＞膈肌＞泌尿生殖道＞脾脏＞肾脏。对通过冲洗回收的细胞进行了细胞离心涂片和流式细胞术的差异计数。与对照组相比，在24小时和48小时从处理过的大鼠中回收的细胞数量增加了约2倍，这主要是由于中性粒细胞流入腹腔所致。与对照组相比，肥大细胞的比例显著降低。
