Giraudon P, Dufay N, Hardin H, Reboul A, Tardy M, Belin M F
CJF 90-10 INSERM, Faculté de Médecine Alexis Carrel, Lyon, France.
Neuroscience. 1993 Feb;52(4):1069-79. doi: 10.1016/0306-4522(93)90553-r.
Constituent cells of medulloblastoma, the most common brain tumor occurring in childhood, resemble the primitive neuroepithelial cells normally found in the developing nervous system. However, mutational events prevent their further differentiation. We used the human T cell lymphotrophic virus type 1 to activate these deregulated immature cells by means of its transactivating protein Tax. Concomitant with viral infection was a decrease in cell proliferation characterized by inhibition of [3H]thymidine incorporation and in the number of cells in the G2/M phase of the cell cycle. Morphological changes suggested that medulloblastoma cells differentiated along the astrocytic lineage. The glial phenotype was confirmed by the induction of the glial fibrillary acidic protein and the glial enzyme glutamine synthetase. A direct viral effect and/or secondary effects to viral infection via paracrine/autocrine pathways could counterbalance the maturational defect in these medulloblastoma cells.
髓母细胞瘤是儿童期最常见的脑肿瘤，其组成细胞类似于正常发育中的神经系统中发现的原始神经上皮细胞。然而，突变事件阻止了它们的进一步分化。我们使用1型人类T细胞嗜淋巴细胞病毒通过其反式激活蛋白Tax来激活这些失调的未成熟细胞。伴随病毒感染的是细胞增殖的减少，其特征是抑制[3H]胸腺嘧啶核苷掺入以及细胞周期G2/M期细胞数量的减少。形态学变化表明髓母细胞瘤细胞沿星形胶质细胞谱系分化。胶质纤维酸性蛋白和胶质酶谷氨酰胺合成酶的诱导证实了胶质表型。病毒的直接作用和/或通过旁分泌/自分泌途径对病毒感染的继发作用可以抵消这些髓母细胞瘤细胞中的成熟缺陷。
